A Systematic Review: Comparison of Immunocytochemistry, ELISA, and Western Blot Methods in Alkaline phosphatase Measurement at Genistein-induced Osteoblast Cell

Osteoporosis is a bone disorder characterized by the decrease of mass along with micro-architecture damage and has risk become fracture. One causes osteoporosis estrogen deficiency. Genistein phytoestrogen compound in isoflavone group containing similar structure compared to 17β-estradiol, thus it can bind receptors produce an estrogenic effect. induction stimulate formation promote increase alkaline phosphate (ALP) activities osteoblast cells which be observed immunocytochemistry or Enzyme-linked Immunosorbent Assay (ELISA) Western blot method. Using PRISMA guideline technique, choose strategize article searches reading title, abstract, then whole text article. Articles keywords "genistein immunofluorescence ELISA western blot" were retrieved from databases including Google Scholar, PubMed, Researchgate, Sciencedirect. 24 relevant research articles uncovered as result this systematic review. Comparison methods order analyze ALP induced genistein includes selectivity, sensitivity, processing time, cost efficiency parameters. The method higher level sensitivity faster whereas selectivity less efficiency. for detecting complex-level protein expression.